Fecha/hora: 14/05/2024 11:32:37

Biblioteca(s): 	INIA Tacuarembó.	
Fecha corriente: 	30/05/2019	
Fecha de la última actualización: 	04/05/2020	
Autoria: 	WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C.	
Afiliación: 	FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.	
Título: 	Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. 

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Año de publicación: 	2019	
Fuente/Imprenta: 	Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837	
DOI: 	10.1002/pca.2837	
Idioma: 	Inglés	
Notas: 	Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química.	
Contenido: 	Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work.	
Palabras-Clave: 	IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS.	
Categoría del tema: 	K11 Ingeniería forestal	
Registro original: 	INIA Tacuarembó (TBO)	
Ejemplares: 	TBO - (102949-1) - PXI - AP - DD -  -  -  - DIS